Plasmid

Part:BBa_I713027:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pBL-FLP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1191
    Illegal XbaI site found at 1958
    Illegal SpeI site found at 873
    Illegal SpeI site found at 1952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1191
    Illegal SpeI site found at 873
    Illegal SpeI site found at 1952
    Illegal NotI site found at 1964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1191
    Illegal BamHI site found at 1946
    Illegal XhoI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1191
    Illegal XbaI site found at 1958
    Illegal SpeI site found at 873
    Illegal SpeI site found at 1952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1191
    Illegal XbaI site found at 1958
    Illegal SpeI site found at 873
    Illegal SpeI site found at 1952
    Illegal NgoMIV site found at 129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3340
    Illegal SapI site found at 2257


Design Notes

BamHI-FLP-XhoI


Source

pBluescript II SK(-)

References